Additionally, to further confirm correlation between expression of p21, pT145-p21 and PI3K/AKT and miR-708-5p expression and metastatic ability, we used immunoblotting to evaluate the expression level of p21, pT145-p21 and pS473-AKT1 in the nine NSCLC cell lines whose expression of miR-708-5p and metastatic ability had been confirmed. The gene discussed is AKT1; the disease is non-small cell lung carcinoma.