Quantification of staining intensity using by Image J (http://openwetware.org/wiki/Sean_Lauber:ImageJ Threshold_Analysis) demonstrated that 20% of the area of tumor stage I samples, 55% in tumor stage II, 80% in tumor stage III, and 90% in tumor stage IV were TLR4-positive, compared to 10% in normal tissue (Figure 1B). This evidence concerns the gene TLR4 and neoplasm.