RHOA and infection: Fig 7A and 7B, shows that mDia1 recruitment to the membrane fraction increased after 30 min and reached its peak after 60 min of infection, a time point after which the levels of protein association decreased to basal levels. The mDia1 showed similar membrane recruitment kinetics when the experiments were performed with heat-killed C. burnetii (S1 Fig). The latter result strongly suggests that mDia1 is activated during C. burnetii-host ́s cell interaction. Interestingly, the infection time of maximum mDia1 recruitment was similar to that observed for RhoA (Fig 3A and 3B).