KIF23 and hepatocellular carcinoma: Immunofluorescence staining and cell fraction analysis with the prepared antibody specific to KIF23 V1, we found that endogenous KIF23 V1 was predominantly localized in the nucleus of the two HCC cell lines (HLE and Huh7), which was consistent with the previous report that CHO1 (KIF23 V1) isoform was present in the nucleus of CHO and HeLa cells [16].