Three of the top four ITRs from this RNAi screen (TNF, TGFB1 and β-estradiol) were also in the top four ITRs of the MYCN amplified versus single copy cell analysis (Figure 6A), revealing a remarkable ability of ITR analysis to identify relevant regulators of neuroblastoma even from highly disparate experimental approaches. Here, TGFB1 is linked to neuroblastoma.