To determine whether melatonin also affect the AP-2/hTERT signaling in NSCLC cells, we treated NSCLC cells with melatonin (1.0 mM) or berberine (20 μM or 100 μM) alone or altogether, and examined expression of AP-2β and hTERT at protein and mRNA levels by Western blot and RT-PCR, respectively. The gene discussed is TFAP2B; the disease is non-small cell lung carcinoma.