TRERF1 and hydrops fetalis: The combination of 2.5 nM HF with 0.1 nM RAPA inhibited 74.56 ± 0.58%, indicating that in the presence of 2.5 nM HF, IC70 of RAPA was the less than 0.1 nM (RAPA alone vs. RAPA + HF: P ˂ 0.0001, two-way ANOVA, n = 5) (Fig 1F).