TRERF1 and hydrops fetalis: Similarly, as shown in Fig 2 addition of proline (1 mM) did not affect RAPA (1 nM)-mediated proliferation inhibition as indicated by no difference between 58.62 ± 5.68% of inhibition by RAPA in the presence of additional proline and 52.68 ± 6.19% in the absence of additional proline (P = 0.1514, n = 5), but it reversed the inhibition of cell growth from 57.12 ± 3.33% to 25.29 ± 4.57% (approximately 57% of recovery) in HF (2.5 nM)-treated cultures (P ˂ 0.0001, n = 5).