TRERF1 and hydrops fetalis: Indeed, RAPA alone at 0.1–100 nM did not induce apoptosis in splenocyte cultures (Fig 3C), but significantly enhanced HF-mediated cell death as indicated by an increase in both annexin-V and 7-AAD stained cells in FACS analysis (Fig 3) or in the expression of cleaved PARP in Western blot using a specific anti-cleaved PARP monoclonal antibody (Fig 4).