TRERF1 and hydrops fetalis: Furthermore, Fig 3C showed that RAPA alone did not affect cell apoptosis in a dose response, indicated by the inhibition from 17.43 ± 5.65% in cultures treated with 0.1 nM RAPA to 19.44 ± 4.76% with 100 nM RAPA (P = 0.0584, one-way ANOVA, n = 4), but in combination with HF the apoptosis was increased to 34.54 ± 3.34% in cultures treated with both 0.1 nM RAPA and 2.5 nM HF, and to 30.61 ± 2.61% with 100 nM RAPA and 2.5 nM HF (RAPA alone vs. RAPA + HF: P ˂ 0.0001, two-way ANOVA, n = 4).