Similarly, RAPA alone inhibited T cell proliferation in a dose-dependent manner (IC70 = 100 nM) (Fig 1 B), and the combination with 2.5 nM of HF also significantly increased the inhibition to 68.58 ± 5.17% in cultures with 0.1 nM of RAPA or 91.04 ± 0.42% with 100 nM of RAPA (IC70 = 0.3 nM, n = 5) (RAPA alone vs. RAPA + HF: P < 0.0001, two-way ANOVA)(Fig 1B). Here, TRERF1 is linked to hydrops fetalis.