A significant increase in risk for gallbladder cancer was observed for larger gallstone (those with stone diameters 2 cm or greater) with the FEN1-69G > A GA (OR = 2.1, 95% CI = 1.0–4.5) and the FEN14150G > T GT (OR = 2.3, 95% CI = 1.0–4.9), whereas significant increases in association for gallbladder cancer were also observed for larger gallstone (those with stone diameters 2 cm or greater) with the FEN1-69G > A GG (OR = 7.3, 95% CI = 1.8–18.8) and the FEN14150G > T GG (OR = 8.2, 95% CI = 1.5–19.8). This evidence concerns the gene FEN1 and gallbladder cancer.