CLN5 and juvenile neuronal ceroid lipofuscinosis: 2002; Frugier et al. 2008; Palmer et al. 2015; Perentos et al. 2015). As with many other forms of Batten disease, mutations in the CLN5 gene have been shown to trigger early synaptic pathology in the affected brain regions of mouse models (von Schantz et al. 2009). We therefore set out to establish the feasibility of isolating synapses from the brain of sheep with Batten disease in order to test whether known molecular regulators of synaptic pathophysiology, previously identified in Drosophila and mouse models, are similarly present and modified in the brain of sheep with CLN5 Batten disease.