To test the hypothesis that altered plasma levels of insulin, leptin, and adiponectin are causative for EI of metabolic disorders in DIO models (Fullston et al. 2013; Ng et al. 2010, 2014; Wei et al. 2014), we induced such hormonal changes in the absence of obesity and most of its complication in F0 male mice by administration of dexamethasone. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.