RNASE1 and infection: To study the viral particles produced by infected cells in the presence or absence of TVB-3166, we harvested cell media 72 hours post infection and eliminated viral RNA that was not encapsidated by digestion with RNase A. Protected RNA was recovered from one portion of the RNase A-treated media, while the level of infectious progeny virions in another portion was determined by titration on Vero cells.