To determine the minimum number of MLL/AF9-OVA leukemia cells required for engraftment in non-irradiated hosts, different numbers (3 × 103, 3 × 104, and 3 × 105) of GFP+ MLL/AF9-OVA leukemia cells were transplanted into non-irradiated recipients, and BM cells were analyzed 1 week after transplant. Here, MLLT3 is linked to leukemia.