In vitro, pretreatment of tumor cells with ACF improved PDT outcome and reduced the PDT-induced expression of VEGF and PTGS2. Lastly, incubation of SK-ChA-1 cells with ACF resulted in induction of S-phase cell cycle arrest, DNA damage, and apoptosis, altogether underscoring ACF's dual topoisomerase I/II inhibition potential and utility to act as a neoadjuvant chemotherapeutic in PDT. Here, VEGFA is linked to neoplasm.