The following 19 clinicopathological factors were analyzed in the training cohort: age, gender, HBsAg, hepatitis C virus antibody (HCV-Ab), alpha-fetoprotein (AFP), alanine aminotransferase (ALT), gamma-glutamyl transferase (γ-GT), liver cirrhosis, Child-Pugh score, tumor differentiation, tumor size, tumor number, tumor encapsulation, vascular invasion, Barcelona Clinic Liver Cancer (BCLC) stage, miR-145, miR-31, miR-92a, and miR-10b. The gene discussed is AFP; the disease is liver cancer.