Nevertheless, using unbiased proteomic analysis, we found that treatment of lung cancer cells with auranofin inhibited phosphorylation of most key nodes in the PI3K/AKT/mTOR pathway, including S6, 4EBP1, Rictor, p70S6K, mTOR, TSC2, AKT, and GSK3, indicating that auranofin may target multiple sites in the PI3K/AKT/mTOR axis. The gene discussed is TSC2; the disease is lung cancer.