Nevertheless, using unbiased proteomic analysis, we found that treatment of lung cancer cells with auranofin inhibited phosphorylation of most key nodes in the PI3K/AKT/mTOR pathway, including S6, 4EBP1, Rictor, p70S6K, mTOR, TSC2, AKT, and GSK3, indicating that auranofin may target multiple sites in the PI3K/AKT/mTOR axis. This evidence concerns the gene AKT1 and lung carcinoma.