TBK1 and non-small cell lung carcinoma: Finally, to test if overexpression of TBK1 compromised microtubule stability, A549 NSCLC cells engineered to overexpress TBK1 (A549-TBK1) and control (A549-GFP) cells were treated with nocodazole (5 μM) for 1 h to depolymerize microtubules; nocodazole was then removed to allow microtubule polymerization26.