In summary, this report describes a novel role of OLA1 (an NTPase) in regulating the interplay between GSK3β (a kinase) and PP1 (a phosphatase), and reveals that OLA1 regulates the balance of protein Ser/Thr phosphorylation and dephosphorylation by suppressing the GSK3β-I-2-PP1 positive feedback loop, a novel signaling motif in the cancer signaling network. Here, GSK3B is linked to cancer.