BRAF and melanoma: Although many different molecular techniques can be used to detect somatic mutations, digital PCR is one of the most sensitive methods that can detect a point mutation when present at levels as low as 0.001–0.01% [25] In one study, digital PCR was compared to high-resolution melt curve analysis, pyrosequencing, and allele-specific amplification to detect the human BRAF V600E mutation in human melanoma tumor tissues [26].