We observed that betatrophin levels did not correlate with β-cell function-related variables (AUC0–0.5, AUC0-1, AUC0–2, and HOMA2-%B) or insulin resistance-related variables (HOMA2-%S and HOMA2-IR) in T2DM subjects before or after controlling multiple covariates. Here, ANGPTL8 is linked to Insulin resistance.