The IQGAP3 expression in 132 primary lung adenocarcinomas (61 from discovery cohort and 71 from validation cohort) with available RNA within the 335 sequenced cases was subsequently measured by quantitative reverse transcription–PCR (RT–PCR; Supplementary Data 17), and confirmed the higher expression level of IQGAP3 in these tumours (Fig. 4a). This evidence concerns the gene IQGAP3 and lung adenocarcinoma.