In addition, using the inguinal lymph node metastasis model, we found that silencing of FLOT2 had no significant effect on the promotion role of FLOT1 in NPC lymph node metastasis in vivo (P > 0.05; Supplementary Figure 6A–6C), suggesting that FLOT1 promoted lymph node metastasis independent of FLOT2 in NPC. Here, FLOT2 is linked to nasopharyngeal carcinoma.