In addition, univariate and multivariate analyses revealed that N classification and FLOT1 expression were each recognized as independent prognostic factors in NPC (both P < 0.05; Supplementary Table 2 and 3), suggesting that FLOT1 has potential clinical value as a predictive biomarker for disease outcome in NPC. This evidence concerns the gene FLOT1 and nasopharyngeal carcinoma.