Histological examination of the primary tumors using H & E staining revealed that the tumors formed by FLOT1-overexpressing CNE2 cells exhibited a more aggressive phenotype, with the FLOT1-overexpressing tumor cells invading towards the muscles, skin and even into the lymphatic vessels, in contrast to the control tumor which showed sharp edges that expanded as spheroids (Figure 3B). Here, FLOT1 is linked to neoplasm.