To further analyse the role of PIM kinases in mediating proliferation and survival of NHL cells, validated shRNAs against PIM1, PIM2 and c-MYC, cloned into the pLKO_IPTG_3xLacO expression vector, were stably transfected into Raji cells and single cell clones were generated. Here, PIM1 is linked to non-Hodgkin lymphoma.