E2F1 and hepatocellular carcinoma: To determine the frequency of H2a.z enrichment at E2f target genes in TKO HCC, we performed chromatin-immunoprecipitation sequencing (ChIP-Seq) analysis with H2a.z and E2f1 pull-down fractions from TKO HCC cells, which resulted in the identification of 10,014 and 3,581 unique hits genome-wide, respectively.