The relative ratio of E2f1 (average of 1.47 ng μl−1) versus E2f3 (average of 2.27 ng μl−1) protein abundance in TKO HCC correlates with the twofold reduction of E2f3 binding over time and E2f1 accumulation (Fig. 7c,d). The gene discussed is E2F3; the disease is hepatocellular carcinoma.