By quantitative Fluorescent Activated Cell Sorting (FACS) analysis, tumor cells treated with TSA or SAHA also showed alterations in differentiation, with depletion of myf5:GFP+/mylz2:mCherry−tumor cell subpopulation and expansion of the late-differentiating mylz2:mCherry+/myf5:GFP−tumor cell subpopulation (results for SAHA treatment shown in Fig 3D). Here, MYF5 is linked to neoplasm.