Similar studies based on the A2/NY-ESO-1157–165 tumor antigen model showed that specific CD8 T cells engineered to express a TCR (defined as 1G4) with affinity lying just beyond the natural affinity range (at KD 730 nM) were those that displayed maximal functionality with the lowest crossreactivity in vitro (59). The gene discussed is CD8A; the disease is neoplasm.