Supporting this, calbindin D-28K immunoreactivity was found reduced in the frontal cortex of patients with FTLD (Ferrer et al., 1993), and in ALS patients, with significant differences in the densities of calbindin positive neurons within cortical layers V and VI (Maekawa et al., 2004). Here, CALB1 is linked to amyotrophic lateral sclerosis.