TRAF6 and alkaline phosphatase measurement: However, results from western blotting analysis clearly showed a decrease in the expression of Traf6 protein in the ANP mice than that in the AEP mice, suggesting that Traf6 is associated with the inflammatory procession of mild AP to severe AP, and the levels of Traf6 was modulated not at the transcriptional level but at the post-translational level.