PFKFB3 and cancer: In addition, we assumed that the amount of glucose in culture medium might affect the regulation of myrAkts on the induced autophagy, as Akt regulates glucose homeostasis with strong isoform specificity.46 Akt stimulates aerobic glycolysis in cancer cells, and activated Akt accelerates cell death upon glucose withdrawal.37 Indeed, here we show that the pharmacologic or genetic inhibition of Akt reduced PFKFB3 expression at both mRNA and protein level.