Notably, all the EGFR and KRAS mutations previously detected by SS were confirmed in all NSCLC samples reporting identical results starting from SF and matched FFPE tissue, although with different allele frequencies due to intra-tumor heterogeneity (Table 1), whereas the other seven genes present in the colon-lung panel showed discordant results between FFPE and matched SF samples. Here, EGFR is linked to non-small cell lung carcinoma.