PRKCD and gastric adenocarcinoma: To investigate whether DAG/PKCδ and IP3/Ca2+/CaMK IIβ were simultaneously involved in regulating cell proliferation and apoptosis in human gastric adenocarcinoma cells, BGC-823 cells were treated with those inhibitors, U73122 (PLCγ inhibitor), KN93 (CaMK II inhibitor) and R59949 (DAG-kinase inhibitor), or were transfected with lentiviral-shRNA of PKCδ or CaMK IIβ vectors for different time points, followed by the detection of cell viability using an MTT assay and apoptotic index using DAPI or PI staining.