In the present study, based on the generalized linear model analysis, the interaction between PEE and HFD increased the serum ACTH, corticosterone, insulin, and glucose levels in the female offspring and decreased the serum triglyceride levels in the male offspring, which suggests that PEE enhanced the susceptibility to MS in the adult female offspring fed a post-weaning HFD. This evidence concerns the gene POMC and myeloid sarcoma.