Supplementary Figure S4 shows that the proliferation of the doubly silenced cells as well as those where each isoform was rescued was modestly affected by the silencing. Conversely, the combined RSK1/RSK2 silencing reduced the ability of the same cell lines to grow in semisolid agar medium (Figure 1D–1E), suggesting a prevailing role of RSK1 and RSK2 in anchorage independent growth of ovarian cancer cells. Individual rescue experiments showed that RSK1 and RSK2 kinases played redundant roles in enabling cell anchorage-independent growth (Figure 1D–1E). The gene discussed is RPS6KA1; the disease is ovarian cancer.