PSAT1 and leukemia: We further documented in immunoblot experiments that the upregulation of PHGDH and PSAT was inversely proportional to the amount of glutamine in the medium (Figure 3C and 3D); we obtained similar results in other leukemia cell lines (Supplementary Figure 2A and 2B) and also when using L-ase (Figure 3E and Supplementary Figure 2C–2D).