IL6 and osteosarcoma: After analysis with ImageJ software, one-way ANOVA and the Newman-Keuls multiple comparison test, we found that osteosarcoma cells (SOSP-9607, F5M2 and F4) expressed higher levels of IL-6 than did normal osteoblastic cells (hFOB 1.19) (P < 0.001, Figure 1b and 1c).