BAX and cancer: In contrast, Bax and Bak suppress cell invasion by inhibiting ROS production; specifically, knockdown/knockout of Bax and Bak in MEFs and various cancer cell types increases basal ROS levels, and prevention of ROS accumulation using antioxidants or metabolic inhibitors abolishes the effects of Bax/Bak knockdown on the PI3K/Akt/MMP-2 pathway and cellular invasiveness [22, 25].