The majority of the causative mutations expected (8 out of 11 LHON-causing mutations) on the LHON dataset was recognized as affecting function by the application of the whole stepwise prioritization workflow with the exception of the mutations m.3700G>A (MT-ND1), m.14502T>C (MT-ND6) and m.14484T>C (MT-ND6) (Supplementary Table 1). The gene discussed is MT-ND6; the disease is Leber hereditary optic neuropathy.