To investigate a potential role of Elovl6 on BA metabolism and whether BA alterations could contribute to reduced LD-induced liver damage and gallstone formation in LD-fed Elovl6−/−Ldlr−/− mice, the composition of individual BAs in the livers of Elovl6+/+Ldlr−/− and Elovl6−/−Ldlr−/− mice fed an SD or an LD were quantified by liquid chromatography/mass spectrometry (LC/MS) (see Supplementary Fig. S2 online). This evidence concerns the gene LDLR and gallstones.