GLI1 and medulloblastoma: In an experiment using RNA-seq designed to identify Shh-responsive genes in mouse embryonic fibroblasts (MEFs), we observed that Hck expression was induced by Shh treatment.39 In a previous study, Hck was identified as a Gli1 binding gene in both CGNPs and medulloblastoma.38 To validate that Hck is a Shh/Gli target gene, we treated Shh-responsive NIH3T3 cells with Shh-conditioned medium.