HCK and medulloblastoma: Previous chromatin immunoprecipitation (ChIP)-on-Chip experiments in CGNP and medulloblastoma identified a potential Gli1 binding site ~1.1 kb upstream of the transcription start site of Hck; the region harbors a Gli binding motif (Figure 1e, Supplementary Figure S1).38 To determine whether Gli1 regulates Hck through direct binding, we performed ChIP experiments using antibodies against HA-Gli1 in NIH3T3 cells.