Given the effect of synaptic vesicle proteins on the pathogenesis of depression and their interaction with anorexigenic or orexigenic neurotransmitters (Fried et al., 1995; Papke et al., 2012; Poretti et al., 2015), one of the aims of the present study is to investigate the potential effect of nesfatin-1 on the expression of synaptotagmin I and synapsin I in the hypothalamus. Here, NUCB2 is linked to major depressive disorder.