We analyzed four independent microarray datasets from the NIH-maintained GEO databases, REMBRANDT, and the TCGA database and found that PLCβ1 gene expression (microarray and RNA-Seq) level is inversely correlated with gliomas’ pathological grades; it is a potential novel signature gene in subclassifying HGG into PN versus other subtypes, and its expression level also correlates with patients’ survival. This evidence concerns the gene PLCB1 and central nervous system cancer.