In addition, the more severe steatosis induced by HCLD is correlated with the levels of the induction of miR-34a, the reduction of SIRT1 protein levels and its activity (as showed by upregulated protein level of ac-FOXO1, the target of SIRT1’s deaceylated acitivity), as well as the reduction of NAMPT. Here, FOXO1 is linked to steatosis.