To assess the onset of aneuploidy, HMEC cells were grown into spheres in the presence of various media combinations such as HMEC media, breast cancer cell (SUM149PT and SUM1315MO2) conditioned media, OPG depleted breast cancer cells conditioned media, and HMEC media supplemented with recombinant human OPG (500 pg/ml or 1100 pg/ml). Here, TNFRSF11B is linked to breast carcinoma.