Considering the low expression level of ROBO1 and ROBO2 in leukaemia cell lines (Supplementary Fig. 7), we transfected the ROBO1 and ROBO2 expression vectors into K562 and HEL cell lines using the SuperFect Transfection system to observe the effect of ROBO1 or ROBO2 on the biological characteristics of leukaemia cells. The gene discussed is ROBO2; the disease is leukemia.