The mutations that were commonly observed together with ROBO mutations included DNMT3A, SF3B1, ANKRD11 and ASXL1. Two cases revealed that both ROBO mutations and SF3B1 had a relatively prolonged survival because of the classification as refractory anemia with ring sideroblast or RCMD-RS. This evidence concerns the gene ANKRD11 and myelodysplastic syndrome with single lineage dysplasia.