Apoptosis was detected 6 hours after pancreatic cancer Panc-28 (Fig 1A) and colon cancer Caco-2 (Fig 1B) cells were dosed with 40 μM of flavone A. In the same manner, apoptosis was detected 6 hours after pancreatic cancer MIA Paca-2 (Fig 1C) and colon cancer HCT116 cells (Fig 1D) were dosed with 40 μM of flavone B. To quantify the apoptotic changes induced by the flavones, cells dosed with the vehicle or flavone, were analyzed 6, 9 and 12 hours after treatment via flow cytometry using Annexin V/ propidium iodide. The gene discussed is ANXA5; the disease is malignant colon neoplasm.