Berberine might induce p53-dependent G1 phase arrest of BEL-7402 cancer cells. After BEL-7402 cells were treated with berberine, five genes (BBC3, FAS, CCNG2, GADD45B, and IGFBP3) were up-regulated and two genes (CASP9, THBS1) were down-regulated (Table C in S1 File) in p53 signaling pathway. The gene discussed is BBC3; the disease is cancer.