However, it has been described that, in the spontaneous B16F10 melanoma cell line, expression of p16Ink4a (inhibitor of CDK4a), which inhibits cell-cycle progression by inactivating cyclin-dependent kinases, and of p19Arf (alternate reading frame tumor suppressor), which causes Mdm2 (mouse double minute 2 homologue) induced translational silencing and p53 degradation, is lost and that there is no evidence of activation of the MAPK-signaling pathway in this cell line [46]. This evidence concerns the gene MDM2 and melanoma.