2011; Breitwieser 2013). The antibody we used for this experiment was against amino acid peptide sequence 15–29 at the extracellular N-terminus of human CaSR (Sigma-Aldrich, St Louis, MO). We observed a similar pattern of staining (multiple bands) when we repeated the experiment using protein extracts from human esophageal biopsies from normal, reflux disease or eosinophilic esophagitis patients or if we used antibodies against other peptide sequences of N or C-termini of the CaSR, confirming that the presence of multiple bands was not an artifact (Fig.7A and B). This evidence concerns the gene CASR and eosinophilic esophagitis.