The present immunohistochemical (IHC) study was conducted to assess the role and relationship of SV40 L-TAG and E2F1 in DLBCL of Egyptian patients, get a hint of whether SV40 and E2F1 are coplayers in this malignancy or not, and correlate the results with the standard clinicopathological and survival data. The gene discussed is E2F1; the disease is diffuse large B-cell lymphoma.