A proteomic analysis to quantify DNA damage-regulated changes in phosphoproteome, acetylome, and proteome in human osteosarcoma cells treated with etoposide showed that a significant fraction of the hits corresponds to proteins involved in RNA metabolism such as THRAP3 (thyroid hormone receptor-associated protein 3), which is part of a multiprotein complex that controls Cyclin D1 mRNA stability, and the splicing-regulator phosphatase protein phosphatase Mg2+/Mn2+ dependent 1G (PPM1G), a nuclear member of the PP2C family of Ser/Thr phosphatases (Beli et al., 2012[5]). Here, THRAP3 is linked to osteosarcoma.