Additionally, there was a highly significant 3-way interaction (category/time-point × vaccination status × FMDV RNA load; p < 0.001): FMDV RNA load had a significant positive influence on IFN-γ and IFN-λ expression in vaccinated animals early after infection (24 hpi; p<0.01 for both), as well as on IFN-λ in non-vaccinated animals that were viremic/pre-clinical (Category II; p<0.001) (Fig 3). The gene discussed is IFNG; the disease is infection.